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Chengbiao Wu, Ph.D
Training:
1994 - Doctor of Biology, Queens University, Kingston Ontario, Canada
1988 - Msc in Biology, Queens University, Kingston Ontario, Canada
1985 - BSc in Biology, Lanzhou University, Lanzhou China
Research Interest:
My studies investigate the role of endogenous Rap1, a small monomeric GTP-binding protein of the Ras family, in nerve growth factor (NGF) signaling . To investigate the molecular basis for Rap1 activation, I examine complexes containing C3G, a guanine nucleotide exchange factor for Rap1, and CrkL, an adapter protein known to influence Rap1 signaling. NGF induces the formation of a long-lived complex containing C3G/CrkL/Shp2/Gab2/TrkA. I have linked the complex to Rap1 activation, by coprecipitated activated TrkA and activated MAPK with activated Rap1 in NGF-treated cells. Confocal microscopy and sub cellular fractionation showed that activated Rap1 and the other proteins of the signaling complex were present in endosomes. We propose that endosomes are a site from which NGF induces the prolonged activation of Rap1 and MAPK. |
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Contact me:
Lab:P220, MSLS building
724-2952
E-mail:cbwu@stanford.edu
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Recent Publication:
Nerve growth factor activates persistent Rap1 signaling in endosomes. J Neurosci. 2001 Aug 1;21(15):5406-16.
Wu C, Lai CF, Mobley WC
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